determined the efficacy of marking the otoliths of three small-sized
progenetic gobies to validate their increment periodicity. These small
gobies have high mortalities and rearing difficulties, making direct
validation difficult. The otoliths were marked by immersing the fish in a
bath of alizarin red S. The fishes were euthanatized and the number of
increments in their otoliths laid down after the fluorescent mark were
counted and compared with the number of elapsed days. The results
validated the daily periodicity of Aphia minuta and Pseudaphya ferreri. The high mortality hindered the validation of Crystallogobius linearis.