DNA extraction from formalin-fixed tissue: new light from the deep sea
Ferran Palero, Sally Hall, Paul F. Clark, David Johnston, Jackie Mackenzie-Dodds and Sven Thatje

DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR success when dealing with formalin-fixed material. Instead, amplification success from formalin-fixed tissue appears to depend on the presence of unmodified DNA in the extracted sample. A staining method that facilitates the targeting of samples with a high content of unmodified DNA is provided.

Keywords: tetramethylsilane, ethidium bromide, formalin, Carcinus, Lithodidae
Contents of this volume Sci. Mar. 74(3) : 465-470 Back PDF
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